摘要 |
PROBLEM TO BE SOLVED: To shorten the measuring time, reduce sample degradation, and reduce the size of obtained data by efficiently obtaining a three-dimensional fluorescence spectrum in a spectrofluorometer. SOLUTION: The spectrofluorometer includes a sample cell for housing a sample components of which are to be analyzed; an excitation-light-side spectrometer for irradiating the sample cell with excitation light having a predetermined wavelength, a fluorescence-side spectrometer for scanning and dispersing the wavelength of the predetermined range of fluorescence from the sample cell, a fluorescence detector for detecting fluorescence from the fluorescence-side spectrometer, and a computer for obtaining a three-dimensional fluorescence spectrum of the intensity of fluorescence of the sample on the basis of the wavelength and intensity of fluorescence detected by the fluorescence detector as changing the wavelength of the excitation light irradiating the sample cell by the excitation-light-side spectrometer. The computer sets a plurality of types of combinations of the range of the wavelength of the excitation light to be dispersed by the excitation-light-side spectrometer and the range of the wavelength of fluorescence to be dispersed by the fluorescence-side spectrofluorometer. COPYRIGHT: (C)2011,JPO&INPIT |