| 摘要 |
The present invention discloses a producing method for tissue engineering cornea. The method involves using amniotic epithelial cells and amniotic stromal cells as seed cells, planting at two sides of decellularized natural corneal stroma after inducement and differentiation in vitro, forming tissue engineering cornea through organ culture in vitro. The scaffold of present invention not only resolves the problem of strong immunological rejection of heterogenetic corneal stroma, but also sustains the structure (which can restore transparency of cornea) and major components (including growth factors which could promote corneal cell growth, proliferation and differentiation) of natural cornea. Compared with the prior art, present invention has advantages of low cost, easy operation, wide range of sources and easy preservation. The said tissue engineering cornea containing live cells has certain elasticity and tenacity, and its shape, size and thickness can be changed easily. The tissue engineering cornea has very low immunogenicity. It could avoid complications caused by non-corneal material, be reconstructed by recipient cells after being transplanted in body, and be incorporated into organism rapidly to become transparent. It could be used to repair corneal wounds caused by all kinds of reasons. |
