| 摘要 |
PROBLEM TO BE SOLVED: To provide new methods to enhance purity of isolated RNA. SOLUTION: A monophase composition, containing phenol for the isolation of purified RNA, can isolate from a biological sample RNA that is substantially free of DNA and thus ready for reverse transcriptase polymerase chain reaction (RT-PCR), contains phenol at final concentration ranging from about 3 to less than 30 wt.% and a buffer in a sufficient amount to maintain the pH of the final composition within the range from about 3.9 to pH 5.5. The isolation method of purified RNA is also provided which uses the composition. COPYRIGHT: (C)2011,JPO&INPIT |
