发明名称 |
Recombinant methylated mycobacterial heparin-binding hemagglutinin antigen |
摘要 |
<p>Immunogenic recombinant peptide (I) is the methylated form of an expression product of a nucleic acid (II) that encodes a mycobacterial heparin-binding hemagglutinin antigen (HBHA), especially from Mycobacterium bovis BCG or M. tuberculosis, is new. Independent claims are also included for: (1) processes for preparing (I); (2) recombinant vector that encodes HBHA and at least one recombinant mycobacterial methyltransferase; (3) host cell that harbors a vector of (2); (4) (I) produced by method (1); and (5) immunogenic composition containing any methylated form of HBHA. ACTIVITY : Tuberculostatic. Mice were immunized with native HBHA (5 mu g), 3 times at 2 week intervals. The titer of HBHA-specific immunoglobulin G (IgG) was then 5000, principally IgG1. Ten weeks after the first immunization, the animals were challenged with 10 5> colony-forming units (cfu) of M. tuberculosis and after 6 weeks the number of cfu in various organs was measured. Compared with adjuvant-only controls, the vaccination caused a reduction, in log10 cfu, of 0.94 in liver; 0.85 in spleen and 0.93 in lungs. Equivalent results (not quoted) were achieved with recombinant methylated HBHA. MECHANISM OF ACTION : Vaccine.</p> |
申请公布号 |
EP2354154(A1) |
申请公布日期 |
2011.08.10 |
申请号 |
EP20100183249 |
申请日期 |
2002.11.18 |
申请人 |
INSTITUT PASTEUR DE LILLE;INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE |
发明人 |
PETHE, KEVIN;MENOZZI, FRANCO;LOCHT, CAMILLE |
分类号 |
C07K14/35;C12N15/09;A61K39/04;A61K39/39;A61P31/06;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12P21/02 |
主分类号 |
C07K14/35 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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