| 摘要 |
FIELD: chemistry. ^ SUBSTANCE: rennet salt extract is prepared and rennet is extracted with sodium chloride solution at temperature 35-40C for 3-3.5 hours, while constantly and slowly stirring without further change of pH. The extract is separated from the raw material and the rennet undergoes activation in the extract at pH 4.6-4.7 by holding it for 8-16 hours at temperature 25-35C. The activated extract is treated with pearlite and then filtered under pressure of 0.015-0.02 MPa at temperature 20-25C. The obtained enzyme is stabilised by adding to the filtrate dry sodium chloride until content thereof in the ready preparation is equal to 17-18%, while increasing pH to 5.3-7.0 with 1n sodium hydroxide solution and adding sodium benzoate until content thereof in the ready product is equal to 0.1-0.5%. ^ EFFECT: optimisation of process conditions for producing rennet in order to achieve maximum activity and purity thereof, simple, cheap and faster process. ^ 2 dwg, 2 ex |
