Method and apparatus for amplifying nucleic acid sequences

摘要

A method of amplifying one or more target nucleic acid sequence(s) in a PCR buffer solution comprising one or more target nucleic acid sequence(s), a nucleic acid polymerase, NTPs, at least a first primer and a second primer is provided. The PCR buffer solution is contained in an elongated vessel (2). The solution within the vessel (2) is locally heated in a spatio-temporally varying manner. Fluid flows (31-34) circulating within the vessel are established. At least a fraction of the PCR buffer solution cyclically travels between regions (35, 36; 37, 38) having different temperatures, giving rise to a temperature cycling.