| 摘要 |
A method for production of single-stranded fragments of DNA, wherein the polymerase chain reaction (PCR) is carried out in two stages: firstly PCR is carried out with two specific primers selected on the basis of sequenced genomic sequences, and double-stranded DNA fragment is produced. After that PCR is carried out with one specific primer, connecting one of the chains by means of DNA-primer into the ring or producing single-double DNA sequences, which are treated with DNA-ligase. Single-stranded DNA-fragments released in such a way are clarified by means of electrophoresis in agarose gel and are isolated by means of TE-buffer. |
