发明名称 |
Method to produce sterile male flowers and partenocarpic fruits by genetic silencing, associated sequences and vectors containing said sequences |
摘要 |
Genes VvPI from Vitis vinifera cv. Cabernet Sauvignon and LePI from Lycopersicon esculentum are described; together with the use of these genes to produce sterile male flowers and seedless or parthenocarpic fruits. Silencing vectors that comprise these sequences or a part thereof are disclosed. The methods of the invention are directed to producing sterile male flowers and parthenocarpic fruits by genetic silencing, and includes: obtaining the codifying sequence of Pistillata (PI)-homologous genes from the target species; analyzing the expression of the sequence obtained in step (a) to test its expression according to the pattern described for Pistillata genes; analyzing the complementation of PI-gene mutant with the PI sequence obtained from the target species, to assess that the obtained sequence fulfills the function of a PI gene; making a genetic silencing construct that comprises a region of the codifying sequence of PI in a plant expression vector; incorporation of the constructed vector into Agrobacterium tumefaciens; transforming target plants with Agrobacterium tumefaciens modified with the silencing vector and selecting said transformed plants; and checking the absence of Agrobacterium contamination and corroborating transgenic plants by transgene amplification.
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申请公布号 |
US7994397(B2) |
申请公布日期 |
2011.08.09 |
申请号 |
US20070948397 |
申请日期 |
2007.11.30 |
申请人 |
PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE |
发明人 |
ARCE JOHNSON JORGE PATRICIO;POUPIN SWINBURN MARIA JOSEFINA;MEDINA AREVALO MARIA CONSUELO;CADAVID LABRADA AGNES;FEDERICI NOE FERNAN |
分类号 |
C12N15/29;C12N15/82 |
主分类号 |
C12N15/29 |
代理机构 |
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代理人 |
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地址 |
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