发明名称 |
THERAPY DELIVERY AND MONITORING USING A GENE OF INTEREST-REPORTER FUSION PROTEIN AND OPTICAL IMAGING |
摘要 |
The present invention relates to a method of non-invasively monitoring the expression of a gene of interest in a cell when contacting said cell with a compound influencing the expression of said gene of interest. The present invention is also concerned with different isolated nucleic acid molecules comprising a coding sequence. Said coding sequence comprises a gene of interest-sequence encoding a gene of interest-polypeptide fused to a reporter sequence encoding a fluorescent reporter polypeptide and is operatively coupled to a promoter sequence. The present invention is also concerned with the use of a method and a nucleic acid molecule of the invention for delivering a compound influencing the expression of a gene of interest in a cell, monitoring the delivery of said compound as well as monitoring the influence on the expression of said gene fluorescence of interest induced by said compound at the same time.
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申请公布号 |
US2011189098(A1) |
申请公布日期 |
2011.08.04 |
申请号 |
US20090993109 |
申请日期 |
2009.05.14 |
申请人 |
KONINKLIJKE PHILIPS ELECTRONICS N.V. |
发明人 |
DIMITROVA NEVENKA;MITAL CHETAN |
分类号 |
A61K49/00;C07H21/00;C12Q1/68 |
主分类号 |
A61K49/00 |
代理机构 |
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