| 摘要 |
A nucleic acid probe set includes (A) a fluorescent probe and (B) a binding probe. The fluorescent probe (A) is formed of an oligonucleotide including (a) a nucleotide labeled with a fluorescent substance. The binding probe (B) is formed of an oligonucleotide having (b1) a fluorescent probe binding region, to which the fluorescent probe (A) can hybridize, and (b2) a target nucleic acid binding region, which can hybridize to a target nucleic acid (C). The nucleic acid probe set is designed such that, when the fluorescent probe (A) hybridizes to the fluorescent probe binding region (b1) and the target nucleic acid (C) hybridizes to the target nucleic acid binding region (b2), guanine in the target nucleic acid (C) and the fluorescent substance (d) labeled on the nucleotide (a) interact with each other to change a fluorescent character of the fluorescent substance (d). The nucleic acid probe can be furnished without needing preparing a fluorescently-labeled, costly nucleic acid probe specifically for every target nucleic acid.
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