| 摘要 |
A DNAzyme is disclosed, which comprises: (i) a catalytic domain with a nucleotide sequence GGCTAGCTACAACGA or a modified sequence of comparable biological effect, which cleaves T-bet mRNA at every purine-pyrimidine binding site to which it is bound; (ii) a right substrate binding domain which is contiguous with the 3′-end of the catalytic domain; and (iii) a left substrate binding domain which is contiguous with the 5′-end of the catalytic domain, whereby the two substrate binding domains are respectively complementary to two regions of the T-bet mRNA so that they hybridize with the mRNA; are active in vivo; and contain the sequences td 69 GGCAATGAA GGC TAGCTACAACGA TGGGTTTCT or td70 TCACGGCAA GGCTAGCTACAACGA GAACTGGGT.
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