发明名称 |
Genome-Wide Construction of Schizosaccharomyces Pombe Heterozygous Deletion Mutants Containing Gene-Specific Barcodes by the Methods of 4-Round Serial or Block PCR, or Total Gene Synthesis Thereof |
摘要 |
A method comprising transforming Schizosaccharomyces pombe with a deletion cassette, constructed by four-round serial PCR, block PCR or total gene synthesis, containing a homologous recombination site is provided for preparing gene-targeted heterozygous deletion Schizosaccharomyces pombe. Also provided are gene-targeted hetero2ygous deletion Schizosaccharomyces pombe mutants prepared by the method, and a library of gene-targeted heterozygous deletion Schizosaccharomyces pombe mutants. Further, the library is useful in constructing a method and a kit for screening a drug's modes of action.
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申请公布号 |
US2011190163(A1) |
申请公布日期 |
2011.08.04 |
申请号 |
US20080989192 |
申请日期 |
2008.08.27 |
申请人 |
KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY |
发明人 |
HOE KWANG LAE;KIM DONG UK;WON MI SUN;YOO HYANG SOOK;KIM DONG SUP;PARK HAN OH;CHUNG KYUNG SOOK;JANG YOUNG JOO;NAM MI YOUNG;HAN SANG JO;CHOI HIN JUNG;BAEK SEUNG TAE;KIM HYONG BAI;HEO KYUNG SUN;LEE HYE MI;LEE MIN HO;PARK JO YOUNG |
分类号 |
C40B30/06;C12N1/19 |
主分类号 |
C40B30/06 |
代理机构 |
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