| 摘要 |
A biological particle sorting device and method of its use. The device includes structure arranged to urge biological particles into substantially single file travel through an interrogation zone. Operable alignment structure nonexclusively include sheathed fluid flow, capillary tubes, an orifice, and fluid microchannels. One or more detector, selected from a plurality of operable such structures, may be employed to sense the presence of a biologic particle in the interrogation zone. Certain exemplary detectors may operate on the Coulter principle, or may detect a Stokes' shift, or side-scatter radiation. Discrimination structure is generally provided to categorize particles as being in one or another sub-population of a mix of biological particles that may be carried in a fluid sample, such as by cell type, size, or the like. Killing structure, such as a laser, is disposed to neutralize all particles in any undesired sub-population while leaving undamaged the desired sub-population(s). The device may be operated to essentially purify (in a living or viable sense) a sample including biological particles that are carried in a fluid diluent.
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