SPECIFIC MOULD DETECTION SYSTEM, SPECIFIC MOULD DETECTION METHOD, AND PCR REACTION LIQUID

摘要

The disclosed specific mould detection system enables rapid, high accuracy detection of specific moulds which often breed in dry environments such as libraries, cultural heritage storage locations, and vaults for a variety of dried industrial goods and foodstuffs. The specific mould detection system has: a PCR reaction liquid containing a pair of primers provided with a primer comprising a base sequence shown in sequence (1) and a primer comprising a base sequence shown in sequence (2), genomic DNA of a sample, a nucleic acid synthetase, and a nucleic acid synthesis substrate; a nucleic acid amplification device which amplifies a nucleic acid by the PCR method using the PCR reaction liquid; and a microarray which identifies the specific moulds by immobilising at least one probe selected from a group comprising probes having a base sequence among the base sequences shown in sequences (3-11) and probes having a complementary base sequence to one of the base sequences shown in sequences (3-11), and hybridising the immobilised probe with the nucleic acid obtained when the genomic DNA of the sample is amplified by the nucleic acid amplification device.