A method for measuring a generation of thrombin in a sample of whole blood as a function of time includes adding to a sample of whole blood a fluorogenic substrate and a thrombin activator to form an activated sample. A conversion product is permitted to form in the activated sample. Fluorescence is measured as a function of time from a fluorescent group that is released during the formation of the conversion product with the use of a fluorescence detector. The fluorescence detector operates in an extended range mode and has an increased sensitivity. Thrombin generation as a function of time can then be calculated from the measured fluorescence as a function of time.
申请公布号
WO2011094185(A1)
申请公布日期
2011.08.04
申请号
WO2011US22344
申请日期
2011.01.25
申请人
ADVENTIST HEALTH SYSTEM/SUNBELT, INC.;FRANCIS, JOHN;COLL, ENRIQUETA;AMIRKOSRAVI, ALI