发明名称 |
Methods for nucleic acid amplification |
摘要 |
Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labelled primers and probes improves reproducibility and quantification. Low-temperature homogenous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utlilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit "single-tube" processing. |
申请公布号 |
EP2351766(A2) |
申请公布日期 |
2011.08.03 |
申请号 |
EP20100187807 |
申请日期 |
2005.10.17 |
申请人 |
BRANDEIS UNIVERSITY |
发明人 |
WANGH, LAWRENCE J.;RICE, JOHN;SANCHEZ, J. AQUILES;PIERCE, KENNETH;SALK, JESSE;REIS, ARTHUR;HARTSHORN, CRISTINA |
分类号 |
C07H21/04;C12P19/34;C12Q1/68 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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