发明名称 |
USE OF NON-NUCLEOPHILIC ADDITIVES FOR REDUCTION OF SURFACE MORPHOLOGICAL ANOMALIES IN PROBE ARRAYS |
摘要 |
The present invention relates to a formulations and methods for coupling a reactant (or probe precursor) to a functionalized surface for purposes of forming an arrayed sensor. This method includes the steps of: providing a surface having a reactive functional group; and introducing onto the surface, at a plurality of discrete locations, two or more compositions of the invention, which include a different reactant (probe precursor) and a non-nucleophilic additive, wherein such introduction is carried out under conditions effective to allow for covalent binding of the reactant to the surface via the reactive functional group. This results in a probe-functionalized array that substantially overcomes the problem of surface morphological anomalies on the array surface. Use of the resulting arrays in various detection systems is also encompassed. |
申请公布号 |
EP2349009(A1) |
申请公布日期 |
2011.08.03 |
申请号 |
EP20090818429 |
申请日期 |
2009.09.30 |
申请人 |
UNIVERSITY OF ROCHESTER |
发明人 |
MACE, CHARLES, R.;YADAV, AMRITA, R.;MILLER, BENJAMIN, L. |
分类号 |
G01N33/543;C12Q1/68 |
主分类号 |
G01N33/543 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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