| 摘要 |
Disclosed herein is a method for immobilisation of a polysaccharide on a detection system carrier, comprising contacting said polysaccharide with an oxidising agent in the presence of a polymer comprising at least two amine and/or amide groups to obtain a polysaccharide-polymer complex and coupling said polysaccharide-polymer complex to said carrier. Also disclosed is a method for determining the presence of a gram-negative bacterium in a sample using said carrier as well as a detection system carrier comprising an immobilised polysaccharide-protein complex on its surface, comprising a coating comprising a carboxyl group donor, preferably a carboxymethylated dextran, linked to a polysaccharide comprising an antigen, wherein said carboxy group donor and said polysaccharide are linked to each other via a polymer comprising at least tow amine and/or amide groups, wherein at least said polysaccharide is linked to said polymer via a periodate oxidised vincinal diol on said polysaccharide and an amine and/or amide group on said polymer.
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