Protein A production and purification without using animal derived components involving staphylococcus aureus

摘要

Disclosed is a non-animal derived Protein A purification process. The process comprises: a) providing a non-animal derived Protein A solution that has been produced by fermenting a secretor strain of Staphylococcus aureus in a media containing vegetarian amino acids or peptides, said media being free of animal products; b) loading the filtered Protein A solution onto a first column; c) washing the column with a buffer-salt mixture to rinse off the column undesirable colour and contaminating proteins; d) eluting the Protein A from the first column to produce a Protein A solution pool; e) adjustment of the pH and conductivity of the Protein A solution pool; f) loading the Protein A from the Protein A solution pool onto a second column; g) washing the second column with a buffer-salt mixture to rinse off the column undesirable colour and contaminating proteins; and h) eluting the Protein A from the second column to produce a purified Protein A solution; wherein the first column is a cation exchange column; and the second column is a column selected from the group consisting of an anion exchange column, a hydrophobic interaction chromatography (HIC) column and a ceramic hydroxyapatite column.