| 摘要 |
PURPOSE: A method for preparing human metabolites of simvastatin or lovastatin using bacterial cytochrome p450 is provided to selectively produce metabolic protucts. CONSTITUTION: A composition for preparing human metabolites of simvastatin or lovastatin has: 47th amino acid arginin(R) of wild type CYP102A1, which is substituted with alanine, valine, leucine, isoleucine, proline, methionine, phenylalanine, or tryptopane; 87th amino acid, phenylalaine(F) which is substituted with alanine, valine, leucine, isoleucine, proline, methionine, or tryptopane; 143th amino acid, gluamic acid(E), which is substituted with glycine, serine, threonine, cysteine, tyrosine, asparagine, or glutamine; and 188th amino acid, leucine(L), which is substituted with glycine, serine, threonine, cysteine, tyrosine, asparagine, or glutamine.
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