| 摘要 |
Disclosed is a polynucleotide encoding, each within a single open reading frame (ORF), at least two polygenes each coding for at least three biologically active polypeptides. At least two of the polypeptides encoded by the genes constituting the polygenes are of non-viral origin, at least two of the polypeptides encoded by the genes constituting the polygenes are each capable of at least transiently interacting with at least one other polypeptide encoded by a gene of said polygenes. The genes constituting each polygene are connected to one another by a sequence coding for at least one protease cleavage site and/or by a sequence coding for at least one self-cleaving peptide. Vectors, host cells, methods of producing the polynucleotide and methods of producing multiprotein complexes are also disclosed. |
