PCR-BASED METHOD OF DISCRIMINATING GINSENG CULTIVARS USING STS PRIMERS

摘要

PURPOSE: A method for determining a STS(sequence tagged site) nucleic acid marker is provided to simply distinguish ginseng kind by genetic pattern. CONSTITUTION: A STS primer set for determining ginseng kind contains: an oligonucleotide primer set of sequence numbers 1 and 2; an oligonucleotide primer set of sequence numbers 3 and 4; an oligonucleotide primer set of sequence numbers 5 and 6; or an oligonucleotide primer set of sequence numbers 7 and 8. The ginseng kind is Chunpoong, Yunpoong, Gopoong, Kumpoong, Sunpoong, or Sunwon. The STS primer set is derived from Panax ginseng C. A. Meyer. A kit for determining the ginseng kind contains the primer set, and reagent for amplification. A method for determining the ginseng kind comprises: a step of isolating genome DNA from a ginseng sample; a step of amplifying a target sequence using the primer set by amplification; and a step of detecting amplified products.