2-PHOTON FLUORESCENT PROBE FOR THIOLS IN LIVE CELLS AND TISSUES

摘要

PURPOSE: A two-photon fluorescent probe is provided to sense thiol in cells and tissues with high selectivity and to enhance fluorescence intensity. CONSTITUTION: A two-photon probe for detecting thiol is denoted by chemical formula 1. The two-photon probe for detecting thion contains 2-methylamino-6-acetylnaphthalene as a reporter and disulfide group as a thiol reaction site. The cells are mouse hippocampus cells. The tissue is mitochondria or nucleus. The depth of the cell or tissue for sensing thiol is 90-180 um.