A cDNA is prepared from mRNA. The prepared cDNA is subjected to incision with two different types of restriction enzymes. The sequence of a portion of each of the fragments obtained as a result of treatment is determined, and the fragments are classified on the basis of the determined sequence by using a primer set. At the same time, the fragments are amplified in a manner that the relative expression magnitude thereof continues to be reflected therein. When the fragments have been classified into a predetermined number of fractions, the respective fractions are subjected to electrophoresis, and a gene expression profile is produced from the results.
申请公布号
CA2431170(C)
申请公布日期
2011.07.26
申请号
CA20012431170
申请日期
2001.12.12
申请人
AISIN SEIKI KABUSHIKI KAISHA;NATIONAL INSTITUTE OF RADIOLOGICAL SCIENCES;MAZE, INC.;ABE, MASUMI;SAITO, TOSHIYUKI