METHOD FOR ANALYSIS OF PROTEINS

摘要

The present invention provides an alternative analysis method to Western blotting which is membrane free without a transfer step, and therefore less time consuming and more convenient than conventional Western blotting. The consumption of affinity ligand is reduced, the detection and quantification is improved. The invention provides a method for analysis of target protein(s) or group of target proteins in a sample, comprising the steps a) enriching each target protein or group of proteins by binding said target protein(s) to an affinity adsorbent having affinity-binding ligand(s) which are directed against said target protein(s) and are immobilized directly to the adsorbent or immobilized via further affinity ligand(s) to said adsorbent,; b) desorbing said target protein(s) from said ligand(s); c) separation of the labeled target protein(s), and d) detection of said target protein(s) wherein said target protein(s) are labeled before or after step a).