发明名称 |
PREPARATION OF AN ESTERASE |
摘要 |
The present invention relates to a recombinant E. coli strain characterized in that the organisms contains a gene coding for a protein having esterase activity. Such E. coli is suitable for the preparation of a protein with esterase activity, whereby the expression takes place without coexpression of GroEL and/or GroES from a plasmid. The expression of glutathione reductase and/or thioredoxin reductase may be abolished by mutation. Furthermore, the ability of the organism to grow under oxygen-rich conditions has been restored by mutation. The expression of the gene of the protein with esterase activity is unaccompanied by the expression of an additional gene encoding a heat shock chaperone protein.
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申请公布号 |
US2011177553(A1) |
申请公布日期 |
2011.07.21 |
申请号 |
US20080667414 |
申请日期 |
2008.07.04 |
申请人 |
KIETZMANN MARTIN;SCHWAB HELMUT;PICHLER HARALD;IVANCIC MIRELA;MAY OLIVER;LUITEN RUDOLF GIJSBERTUS MARIE |
发明人 |
KIETZMANN MARTIN;SCHWAB HELMUT;PICHLER HARALD;IVANCIC MIRELA;MAY OLIVER;LUITEN RUDOLF GIJSBERTUS MARIE |
分类号 |
C12P21/02;C12N1/21;C12N15/55;C12N15/70;C12P7/40 |
主分类号 |
C12P21/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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