发明名称 |
OLIGONUCLEOTIDES AND METHODS FOR DETECTING KRAS AND PIK3CA MUTATIONS |
摘要 |
Provided are oligonucleotides that are capable of detecting KRAS and PIK3CA mutations in both cancer patients and healthy individuals with high specificity in kPCR assays. When the oligonucleotides are used as forward primers in conjunction with a defined genotyping algorithm spreadsheet, the primers are capable of enhancing detection of KRAS codon 12, 13, and 61 and PIK3CA codon 542, 545, and 1047 single nucleotide polymorphisms (SNPs) in a background of wild-type sequences. The oligonucleotides of the present invention are also capable of preventing pseudogene amplification when the oligonucleotides are hybridized as reverse primers or detection probes to the mismatch sequences. |
申请公布号 |
WO2011087928(A1) |
申请公布日期 |
2011.07.21 |
申请号 |
WO2011US20098 |
申请日期 |
2011.01.04 |
申请人 |
SIEMENS HEALTHCARE DIAGNOSTICS INC.;DETMER, JILL;UZGIRIS, AREJAS;YING, ANDY |
发明人 |
DETMER, JILL;UZGIRIS, AREJAS;YING, ANDY |
分类号 |
C07H21/00;C12P19/34;C12Q1/68 |
主分类号 |
C07H21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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