SYSTEMS AND METHODS FOR IDENTIFYING MICROPARTICLES

摘要

Disclosed are systems and methods for identifying microparticles or features arranged in high density arrays. Using the techniques of the present teachings allow for effective discrimination and characterization of microparticles such as sequencing beads or features having densities of about 39 x 106 particles/cm2 or more. In certain embodiments, such identification can be achieved via use of two or more images corresponding to respective subsets of the microparticles or features. In certain embodiments, microparticles in each such subset can be configured with a target that hybridizes with a labeled probe, thereby resulting in the corresponding image having lower density of objects to identify. The method of microparticle identification may encompass comparing of subset images with a common image, ranking of the microparticles to select those with the highest fluorescent identity. The invention further encompasses a system for biological analysis comprising a flow cell for receiving microparticles and a computer readable medium with instructions for identifying and ranking the microparticles.