CONJUGATED CHAIN BETA-N-SUBSTITUTED PENTAMETHINE CYANINE FLUORESCENT DYE

摘要

A conjugated chain beta-N-substituted pentamethine cyanine fluorescent dye is provided. The dye reacts with biological matrix including protein, liposome and DNA, and shows excellent selectivity. It has good living cell membrane permeability, and has good selectivity in living cell staining. In addition, the dye has good stability and low background fluorescence, and can be used together with other fluorescent dyes having consistent wavelengths for single-channel excitation and multi-channel detection. The dye is used in the fields of protein labeling and detecting, fluorescence immunoassay and living cell selective imaging and the like.