| 摘要 |
<p>The present invention discloses methods of neutralizing apatite surfaces, for example during chromatography and before protein elution. Specifically, provided are methods for purifying a target molecule in a sample, comprising contacting a sample comprising the target molecule to an apatite solid surface, contacting the solid surface comprising the adsorbed target molecule with a solution comprising a basic amino compound and alkali metal ion or alkali earth ion; or a sulphonated amine compound and alkali metal ion or alkali earth ion, and eluting the target molecule from the solid support by contacting the solid support with a different solution.</p> |
