DETECTION OF NUCLEIC ACID SEQUENCE DIFFERENCE USING COUPLED LIGASE DETECTION AND POLYMERASE CHAIN REACTION

摘要

PROBLEM TO BE SOLVED: To provide a method for detecting the difference of detected sequences.

SOLUTION: The invention relates to the detection of nucleic acid sequence differences using coupled ligase detection reaction (LDR) and polymerase chain reaction (PCR). One aspect of the invention involves use of a ligase detection reaction coupled to a polymerase chain reaction. Another aspect of the invention relates to the use of a ligase detection reaction coupled to a secondary polymerase chain reaction coupled to primary polymerase chain reaction. A third aspect of the invention involves a secondary polymerase chain reaction coupled to a primary polymerase chain reaction. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.

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