| 摘要 |
A method for diagnostic analyses, in particular to identify pathogens, such as viruses, bacteria or other micro-organisms present in a biological sample, comprises a first step of measuring and continuously monitoring the turbidity and/or the concentration of the pathogens, by means of an instrumental reading technique, of a liquid culture medium into which the sample to be analyzed has been inoculated and in which the replication of the pathogens possibly present occurs, said measuring and monitoring being carried out dynamically during the replication of the pathogens growing in the culture medium; and a second step of identifying the pathogens, carried out by taking at least an aliquot of the liquid culture medium containing the biological sample directly obtained from the first step, which has reached a desired value of turbidity according to a standardized value scale, such as the McFarland turbidity scale, and/or of concentration of the pathogens, and using said aliquot directly in mass spectrophotometric identification means (15) in order to identify the pathogens, which means are calibrated in their functioning depending on the measurement results of the first step. The desired values of turbidity and/or of concentration of the pathogens are preliminarily selected, during the first step, on the basis of the specific needs which, on each occasion, are identified in order to carry out the second identification step |
