METHOD OF SPECIFIC ASCARIS LUMBRICOIDES DETECTION IN CLINICAL MATERIAL IN VARIOUS STAGES OF DISEASE

摘要

FIELD: medicine. ^ SUBSTANCE: total DNA is recovered from an analysed material. The analysed material is excrement, mucus, sputum, blood, section material. The method involves specific amplification of an 18S gene fragment of rRNA of Ascaris lumbricoides with the use of specific primers. If the electrophoresis detection shows an amplification product of the size 489 pn, an agent is considered to be detected. ^ EFFECT: use of the invention allows objectively detecting a fact of human Ascaris lumbricoides infection and diagnosing ascariasis regardless of a type of the analysed material and a stage of the disease. ^ 2 ex