| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for measuring TTF-1 mRNA, having good reproducibility, suitable for the treatment of multispecimen, capable of reducing an inspection cost, and hardly causing secondary pollution of an amplified nucleic acid. <P>SOLUTION: The method for measuring the TTF-1 mRNA includes measuring the amount of amplified RNA products with time by an oligonucleotide probe designed so that the signal characteristics may change when a complemental double-stranded chain is formed with the amplified RNA in an RNA amplification process comprising a step for forming a double-stranded DNA including a promoter sequence by using a combination of oligonucleotides comprising a first primer having a sequence homologous to a part of the TTF-1 mRNA, and a second primer having the complementary sequence (a promoter sequence is added to the 5' terminus of one of the first and second primers) by a reverse transcription enzyme, forming an RNA transcription product by an RNA polymerase by using the double-stranded DNA as a template, and successively forming the double-stranded DNA by using the RNA transcription product as the template of the DNA synthesis by the reverse transcription enzyme. <P>COPYRIGHT: (C)2011,JPO&INPIT |
