发明名称 |
ASSEMBLY OF HIGH FIDELITY POLYNUCLEOTIDES |
摘要 |
Methods and apparatus relate to the synthesis of high fidelity polynucleotides and to the reduction of sequence errors generated during synthesis of nucleic acids on a solid support. Specifically, design of support-bound template oligonucleotides is disclosed. Assembly methods include cycles of annealing, stringent wash and extension of polynucleotides comprising a sequence region complementary to immobilized template oligonucleotides. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products. |
申请公布号 |
WO2011085075(A2) |
申请公布日期 |
2011.07.14 |
申请号 |
WO2011US20335 |
申请日期 |
2011.01.06 |
申请人 |
GEN9, INC.;JACOBSON, JOSEPH;CHU, LARRY LI-YANG |
发明人 |
JACOBSON, JOSEPH;CHU, LARRY LI-YANG |
分类号 |
C12N15/10 |
主分类号 |
C12N15/10 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|