METHOD FOR DETECTING AND IDENTIFYING SPECIES OF THE FAMILY ENGRAULIDAE

摘要

Method for detecting and identifying species of the family Engraulidae. The present invention relates to a method for the simultaneous detection and identification in a sample of at least one of the species of the family Engraulidae selected from Engraulidis encrasicolus, Engraulis mordax and Engraulis ringens, by multiplex PCR amplification of at least one of the sequences selected from SEQ ID NO 1, SEQ ID NO 2 and SEQ ID NO 3, corresponding to the non-transcribed spacer (NTS) of the 5S ribosomal DNA of at least one of the 3 species, using at least one of the pairs of sequence primers selected from: SEQ ID NO 4 and SEQ ID NO 5; SEQ ID NO 6 and SEQ ID NO 7; and SEQ ID NO 8 and SEQ ID NO 9.