MUTANT C-CSF PRODUCTION WITH INCREASED BIOLOGICAL ACTIVITY

摘要

PURPOSE: A mutant human G-CSF with increased biological activity is provided to ensure superior biological activity and excellent economic effect with low dosage. CONSTITUTION: A mutant human G-CSF has amino acid modification by N-glycosylateion by formation of Asn-X-Ser/Thr(N-X-S/T) sequence in an amino acid residues: T1-P10 (T1-P-L-Q-P-A-S-S-L-P10); K40-Q70 (K40-L-C-H-P-E-E-L-V-L-L-G-H-S-L-G-I-P-W-A-P-L-S-S-C-P-S-Q-A-L-Q70); or L124-S142 (L124-G-M-A-P-A-L-Q-P-T-Q-G-A-M-P-A-F-A-S142). A pharmaceutical composition contains the mutant human G-CSF.