LUMINESCENCE MICROSCOPY

摘要

The invention relates to a luminescence microscopy method, including: a sample (P) being used, which comprises a certain substance, or the sample is provided with said certain substance, wherein the certain substance can be converted repeatedly from a first state (A), in which it can be excited into emitting luminescence radiation (3), into a second state (B), in which it cannot be excited into emitting luminescence radiation; the certain substance present in the sample being brought into the first state by irradiating switch radiation (1); the certain substance present in the sample being excited into emitting luminescence radiation by irradiating excitation radiation (2); and the sample emitting luminescence radiation being displayed, wherein a high-resolution selection of sample regions extending perpendicularly to a sample surface is carried out by irradiating either the switch radiation or the excitation radiation as structured illumination (6) of the sample, and wherein a high-resolution selection of the sample surface is carried out by irradiating the switch radiation and/or the excitation radiation as TIRF illumination of the sample.