发明名称 |
DETECTION AND QUANTIFICATION OF BIOMOLECULES USING MASS SPECTROMETRY |
摘要 |
The present invention is directed in part to a method for detecting a target nucleic acid using detector oligonucleotides detectable by mass spectrometry. This method takes advantage of the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed oligonucleotide probes from hybridized duplexes and releases labels for detection by mass spectrometry. This process is easily incorporated into a polymerase chain reaction (PCR) amplification assay. The method also includes embodiments directed to quantitative analysis of target nucleic acids.
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申请公布号 |
US2011160093(A1) |
申请公布日期 |
2011.06.30 |
申请号 |
US201113040056 |
申请日期 |
2011.03.03 |
申请人 |
SEQUENOM, INC. |
发明人 |
VAN DEN BOOM DIRK JOHANNES;OETH PAUL ANDREW;MAHBOUBI PAYAM |
分类号 |
C40B40/06;C07H21/00 |
主分类号 |
C40B40/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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