发明名称 |
Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases |
摘要 |
Disclosed herein are methods and compositions for inactivating TCR genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain in conditions able to preserve cell viability. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided. Disclosed herein are also methods and compositions for expressing a functional exogenous TCR in the absence of endogenous TCR expression in T lymphocytes, including lymphocytes with a central memory phenotype. Polynucleotides encoding exogenous TCR, vectors comprising polynucleotides encoding exogenous TCR and cells comprising polynucleotides encoding exogenous TCR and/or cells comprising exogenous TCR are also provided.
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申请公布号 |
US2011158957(A1) |
申请公布日期 |
2011.06.30 |
申请号 |
US20100927292 |
申请日期 |
2010.11.10 |
申请人 |
SANGAMO BIOSCIENCES, INC.;FONDAZIONE CENTRO SAN RAFFAELE DEL MONTE TABOR |
发明人 |
BONINI MARIA CHIARA;GENOVESE PIETRO;GREGORY PHILIP D.;HOLMES MICHAEL C.;NALDINI LUIGI;PASCHON DAVID;PROVASI ELENA;ZHANG LEI |
分类号 |
A61K35/12;A61P31/00;A61P35/00;A61P37/06;C07H21/04;C12N5/00;C12N5/07;C12N9/16;C12N15/63 |
主分类号 |
A61K35/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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