METHOD FOR DETECTION OF RNA MOLECULE, KIT AND USE RELATED THERETO

摘要

PROBLEM TO BE SOLVED: To provide an improved detection method for a low RNA molecule. SOLUTION: The method for the detection of an RNA molecule comprises: (a) a step of providing a sample containing the RNA molecule; (b) a step of hybridizing a first polynucleotide to the RNA molecule; (c) a step of reversely transcribing the RNA molecule to generate a first strand cDNA; (d) a step of hybridizing a second polynucleotide to the first strand cDNA, wherein the second polynucleotide is 3'-inextensible oligonucleotide containing (i) 3'-moiety complementary to a part of the first strand cDNA and (ii) 5'-projection containing the sequence of a second primer binding site; (e) a step of obtaining an extension reaction product containing a sequence complementary to the second primer binding site from the first strand cDNA; (f) a step of amplifying the extension reaction product by using the second primer complementary to the first primer and second primer binding sites and by means of polymerase chain reaction; and (g) a step of detecting the amplification product by means of real-time fluorescence readout. COPYRIGHT: (C)2011,JPO&INPIT