RECOMBINANT HOST FOR PRODUCING L-ASPARAGINASE II

摘要

Disclosed is a recombinant Escherichia coli host cell for producing an Escherichia coli L-asparaginase II enzyme, comprising an Escherichia coli chromosome and at least one copy of a recombinant extrachromosomal vector, wherein the recombinant extrachromosomal vector encodes a subunit of the L-asparaginase II enzyme, wherein the host cell chromosome also encodes the same subunit of the L-asparaginase II enzyme, and wherein the host chromosome does not encode any other isoform of L-asparaginase II, wherein the encoded L-asparaginase II subunit comprises SEQ ID NO: 1; and the recombinant extrachromosomal vector comprises a DNA molecule encoding the L-asparaginase protein II subunit comprising SEQ ID NO: 2.