发明名称 |
REACTOR FOR BULK MANUFACTURE OF PCR AMPLICONS |
摘要 |
A reactor and methods of use are disclosed for amplification of an amplicon. The reactor includes a housing having a fluid path with a fluid inlet and a fluid outlet and one or more reaction chambers suitable for housing the amplicon. The reaction chambers are positioned within the housing in thermal communication with a fluid flowing in the fluid path to heat and/or cool the reaction chambers and the inside of the reaction chambers are coated with or are a plastic material. A variable temperature fluid source is in fluid communication with the fluid inlet and the fluid source is configured to provide fluid at temperatures suitable for amplification, including a denature temperature, an anneal temperature, and an extension temperature. A controller is coupled to the variable temperature fluid source to control a fluid temperature within the fluid path by controlling the variable temperature fluid source.
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申请公布号 |
CA2775074(A1) |
申请公布日期 |
2011.06.30 |
申请号 |
CA20102775074 |
申请日期 |
2010.10.01 |
申请人 |
VICAL INCORPORATED |
发明人 |
MANTHORPE, C. MARSTON;MINICK, MARK E.;DOMANICO, EDWARD M. |
分类号 |
C12M1/38;C12M1/00;C12P19/34;C12Q1/68 |
主分类号 |
C12M1/38 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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