Herein is reported a method for reducing the aggregation of an immunoglobulin in solution comprising the steps of i) comparing the amino acid sequence of the fourth framework region of the heavy chain of an antibody with a reference or germline sequence and determining whether one or more threonine residues and/or serine residues have been replaced by a different amino acid residue, and ii) modifying the amino acid sequence of the immunoglobulin by reverting the exchanged threonine residues and/or serine residues back to threonine or serine of the reference or germline sequence and thereby reducing the aggregation of an immunoglobulin in solution.
申请公布号
WO2011076684(A1)
申请公布日期
2011.06.30
申请号
WO2010EP70063
申请日期
2010.12.17
申请人
F. HOFFMANN-LA ROCHE AG;KETTENBERGER, HUBERT;KLOSTERMANN, STEFAN;KOHNERT, ULRICH;NEUMANN, SEBASTIAN
发明人
KETTENBERGER, HUBERT;KLOSTERMANN, STEFAN;KOHNERT, ULRICH;NEUMANN, SEBASTIAN