| 摘要 |
The present invention relates to the real-time multiplex detection of at least three target nucleic acid sequences with elimination of false positive signals. Unlikely to conventional real-time multiplex PCR methods, the present invention comprises two different amplification reactions in different reaction vessels from each other: a primary multiplex PCR for obtaining amplicons and a secondary nested real-time multiplex PCR using the amplicons. The present invention permits to eliminate the false positive signals generated by the dimer formation of labeled primers, false positive signals from non-specific hybridization, and false negative signals. |
