The present invention relates to a method for producing a product of a reaction catalysed by a nitrilase, which method comprises the steps (i) providing a microorganism comprising said nitrilase located on its surface, and/or a membrane preparation of said microorganism, and (ii) contacting the microorganism and/or the membrane preparation thereof with one or more nitrilase substrates under conditions compatible with nitrilase activity. The present invention further relates to a method for producing enantiomerically pure (R)-mandelic acid using the nitrilase-displaying whole cell biocatalyst or membrane preparation thereof for the conversion of racemic mandelonitrile.
申请公布号
EP2338987(A1)
申请公布日期
2011.06.29
申请号
EP20090014294
申请日期
2009.11.16
申请人
ZYRUS BETEILIGUNGSGESELLSCHAFT MBH & CO. PATENTE I KG