DUPLEX PRIMERS FOR DIAGNOSING INSV AND TSWV, AND USES THEREOF

摘要

PURPOSE: A duplex primer for diagnosing INSV and TSWV is provided to specifically diagnose INSV and TSWV and to enhance detection limit. CONSTITUTION: A duplex primer for diagnosing INSV(impatiens necrotic spot virus) and TSWV(tomato spotted wilt virus) contains oligonucleotide primer set containing an oligonucleotide with 15 or more continuous nucleotide fragment in sequence number 1 and 15 or more continuous nucleotide fragment in sequence number 2. A kit for simultaneously diagnosing INSV and TSWV contains the primer set and reagent for reverse-transcription and amplification.