摘要 |
PURPOSE: An artificial cultivation method of mycoleptodonoides aitchisonii is provided to mass-produce the mycoleptodonoides aitchisonii containing useful components. CONSTITUTION: An artificial cultivation method of mycoleptodonoides aitchisonii comprises the following steps: mixing 100parts of liquid or solid inoculation medium by weight, 0.3~3parts of carbon source by weight selected from maltose or sucrose, 0.02~0.2parts of nitrogen source by weight selected from asparagines or sodium nitrate, and 0.02~2parts of mineral by weight selected from potassium diphosphate or magnesium sulfate; injecting spawns of mycoleptodonoides aitchisonii strain into the mixture, and cultivating in a bottle medium form; preparing a nutrient medium containing broad-leaf tree sawdust, rice bran, brewers grain, and other ingredients; inserting the bottle medium with the spawns into a cultivation chamber, and cultivating in 18~28deg C; low temperature treating the bottle medium for 6~12days; and growing the mycoleptodonoides aitchisonii by high temperature treating the bottle medium for 15~25days. |