RECOMBINANT PLASMID, Escherichia coli STRAIN, CHIMERIC PROTEIN AND THEIR APPLICATION

摘要

FIELD: medicine. ^ SUBSTANCE: recombinant plasmid pCFP10-CBD consisting of an artificial bacterial operon of chimeric protein containing a promotor area of an early promotor of bacteriophage T5, chimeric protein gene and transcription terminator, a bacterial operon of beta-lactamase and a bacterial ColEl-type replication initiation site is described. The invention also involves the Escherichia coli strain - producer of chimeric protein CFP10-CBD, and also a method of immobilisation, concentration and cellulose purification of produced protein. Besides, the invention refers to the same recombinant protein CFP10-CBD and an immunogenic composition containing it and used for tuberculous infection immunity induction. ^ EFFECT: invention allows producing the strain - producer providing a high production level of resistant immunogenic proteins which can be one-stage produced, immobilised and purified, producing the effective immunogenic compositions for tuberculosis. ^ 5 cl, 1 dwg, 4 ex