发明名称 |
METHOD FOR QUICK DETECTION, ISOLATION AND COUNT OF APOPTOTIC CELLS THAT IS BASED ON AGGLUTINATION |
摘要 |
The present invention relates to methods and kits for the detection, isolation and quantification of apoptotic cells based on the apoptotic cells' increased expression of alpha-D-mannose and/or beta-D-galactose containing glycoproteins. Lectins that bind to alpha-D-mannose and beta-D-galactose-rich glycoconjugates are used in the methods and kits for agglutination tests for the detection, isolation and quantification of apoptotic cells. Lectins may be used to stimulate the agglutination of cells and apoptosis may be detected by assessing the concentration of lectins required to agglutinate a cell population and comparing the concentration to predetermined values for intact cells and cells in various stages after induction of apoptosis. |
申请公布号 |
UA94920(C2) |
申请公布日期 |
2011.06.25 |
申请号 |
UA20080007435 |
申请日期 |
2006.10.31 |
申请人 |
INSTITUTE OF CELL BIOLOGY OF THE NATIONAL ACADEMYOF SCIENCES OF UKRAINE |
发明人 |
STOIKA ROSTYSLAV STEPANOVYCH;BILYI ROSTYSLAV OLEKSANDROVYCH;ANTONIUK VOLODYMYR OLEKSANDROVYCH |
分类号 |
G01N33/53 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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